Top Oligonucleotide Synthesis Companies

Published: April 2024


Due to long development timelines, lack of purification techniques for oligonucleotides synthesized, regulatory and compliance-related issues, and lack of expertise, developers in the oligonucleotide synthesis market prefer to outsource their oligonucleotide manufacturing operations to contract service providers. We analyzed more than 110 industry players that offer synthesis services for oligonucleotide therapeutics. In this article, discover top 5 Oligonucleotide Synthesis Service Providers.

Oligonucleotides (commonly referred to as oligos) are short single stranded DNA or RNA molecules, typically comprising of 15-20 nucleotide residues. These molecules are widely used in biopharmaceutical and biotechnology industries for a wide range of applications, including research and genetic testing, forensic analysis and other analytical procedures. Presently, researchers are actively exploring the use of oligonucleotides as a pharmacological intervention, for the treatment of a myriad of disease indications.  In fact, post the approval of the first antisense oligonucleotide drug in 1998, a number of such candidates have progressed into late-stage clinical trials. As a result, the demand for oligonucleotide-based products has increased significantly in the last few years.

Oligonucleotides are used in a variety of applications, ranging from polymerase chain reaction (PCR) and sequencing to carrying out mutagenesis and single-nucleotide polymorphism assays. These molecules can be manufactured synthetically as per the customizations and specific needs of the customers.  Oligonucleotide synthesis involves creating of short segments of nucleic acids with specific sequences. Over the past few decades, the process of oligonucleotide synthesis was quite inefficient and labor intensive. The growing need for a significant volume of oligonucleotide material for therapeutic and research applications has prompted the development of more economically efficient manufacturing methods. Since the field of oligonucleotides is relatively niche in terms of commercial applications, it lacks decades-long experience in large-scale manufacturing. Historically, much of the oligonucleotide advancement occurred within laboratory settings. However, with the rapid expansion of the field, manufacturers are shifting from laboratory-based production to more scaled-up manufacturing processes.

Roots Analysis has conducted an exhaustive study on Oligonucleotide Synthesis Market featuring the current market landscape and future opportunity associated with the oligonucleotide synthesis domain, over the forecast period, till 2035.

Table of Contents

Below, we have listed (in alphabetical order), the top 5 oligonucleotide synthesis companies, based on their service portfolio / capabilities.

Company    YoE     HQ       Employee Count
Agilent Technologies 1999 Agilent Technologies - Headquarter 10,000+
Ajinomoto Bio-Pharma Services  1989 Ajinomoto Bio-Pharma Services - Headquarter 1,001-5,000
Integrated DNA Technologies 1987 Integrated DNA Technologies - Headquarter 1,001-5,000
Merck 1668 Merck - Headquarter 10,000+
Nitto Avecia 2011 Nitto Avecia - Headquarter 501-1,000

Interested in exploring all 110+ oligonucleotide synthesis service providers?

By leveraging its technical knowledge and operational expertise, Agilent Technologies provides contract development and manufacturing services to support the commercialization of oligonucleotide APIs

Agilent Technologies - Logo

Agilent Technologies is a US-based company that specializes in offering application focused solutions, including instruments, software, services and consumables for various laboratory purposes; and manufacturing of several advanced analytical, research, and diagnostic solutions and tools. The firm operates through three business segments, including Life Sciences and Applied Markets, Diagnostics and Genomics, and Agilent CrossLab.

The following table presents the details on the services offered by Agilent Technologies:

Parameter Description 
Number of Facilities 2
Location of Facilities Colorado, US
Type of Oligonucleotide Synthesized 
  • Antisense Oligonucleotides
  • Aptamers
  • DNA Oligonucleotides
  • Oligonucleotide Probes
  • RNA Oligonucleotides (gRNA, miRNA, shRNA and siRNA)
Scale of Operation
  • Preclinical
  • Clinical
  • Commercial
Application of Area
  • Research
  • Therapeutic
Type of Product Synthesized  Oligonucleotide API
Type of Additional Services Offered
  • Process Development / Method Validation
  • Analytical Testing
  • Purification
  • Quality Assurance and Control
  • Regulatory Support


Recent Initiatives Undertaken by Agilent Technologies:

  • In March 2023, the company acquired e-MSion to complement its growing biopharma solutions, including oligonucleotide analysis software.
  • In January 2023, the company expanded its oligonucleotide manufacturing capabilities through an investment of USD 725 million at its facility based in Colorado, US.

Ajinomoto Bio-Pharma Services is engaged in offering high quality custom oligonucleotides, with its innovative AJIPHASE  synthesis technology

Ajinomoto Bio-Pharma Services - Logo

Ajinomoto Bio-Pharma Services is a US based contract development and manufacturing organization with over 100 manufacturing facilities and offices across 35 different nations, worldwide.  The company provides a wide portfolio of development, cGMP manufacturing and aseptic fill / finish services for the production of small and large molecules. In addition, the company offers services related to ADCs, HPAPIs, oligonucleotides, continuous flow manufacturing, and protein expression technology, at preclinical, clinical and commercial scales.

The following table presents the details on the services offered by Ajinomoto Bio-Pharma Services:

Parameter Description 
Number of Facilities 1
Location of Facilities Tokai, Japan
Type of Oligonucleotide Synthesized 
  • Antisense Oligonucleotides
  • Aptamers
  • DNA Oligonucleotides
  • RNA Oligonucleotides (miRNA, siRNA, Decoy RNA)
Scale of Operation
  • Preclinical
  • Clinical
  • Commercial
Application of Area
  • Research
  • Diagnostics
  • Therapeutics
Type of Product Synthesized Oligonucleotide API
Type of Additional Services Offered
  • Process Development / Method Validation
  • Analytical Testing
  • Custom Synthesis
  • Sequencing
  • Modification
  • Purification
  • Quality Assurance and Control


Recent Initiatives Undertaken by Ajinomoto Bio-Pharma Services:

  • In February 2022, the company developed a new highly functional artificial RNA ligase enzyme for oligonucleotide synthesis.

With over 35 years of experience IDT is dedicated at offering highest standards of quality, technical expertise and personalized customer services to their end users

Integrated DNA Technologies - Logo

Integrated DNA Technologies (IDT) is a US based company that specializes in the design, manufacture and distribution of high-quality DNA and RNA oligonucleotides, across the globe. In addition, IDT claims to be an innovative diagnostic solution provider for oncological disorders, infectious diseases and other complex diseases. Further, the firm is focused on providing proprietary synthesis platforms, processing technology, automation, rapid prototyping and custom reagent production offerings. In a rapidly evolving technological landscape, IDT meets the challenges of translating research from the lab to real-world applications, offering expanded production facilities with GMP and cGMP manufacturing grades.

The following table presents the details on the services offered by Integrated DNA Technologies:

Parameter Description 
Number of Facilities 7
Location of Facilities
  • California, US
  • Iowa, US
  • Michigan, US
  • North Carolina, US
  • Colorado, US
  • Leuven, Belgium
  • Singapore
Type of Oligonucleotide Synthesized 
  • Antisense Oligonucleotides
  • Aptamers
  • DNA Oligonucleotides
  • Oligonucleotide Probes
  • Primers
  • RNA Oligonucleotides (miRNA, siRNA)
Scale of Operation
  • Preclinical
  • Clinical
  • Commercial
Application of Area
  • Research
  • Diagnostics
  • Therapeutics
Type of Additional Services Offered
  • Process Development / Method Validation
  • Analytical Testing
  • Custom Synthesis
  • Modification
  • Purification
  • Quality Assurance and Control


Recent Initiatives Undertaken by Integrated DNA Technologies:

  • In October 2023, the company expanded its oligonucleotide manufacturing capabilities through the opening of a new facility in Iowa, US.

Merck provides DNA oligos, RNA oligos, predesigned primers and qPCR probes for various research and diagnostic applications, such as PCR, cloning, next-generation sequencing, microarray production, CRISPR, gene synthesis, gene expression analysis and pathogen detection

Merck - Logo

Merck is a leading science and technology company, operating through three primary business segments, namely healthcare, life sciences and performance materials.  The healthcare segment of the company focuses on developing novel therapies and medical technologies, for different therapeutic areas, including allergen immunotherapy, endocrinology, general medicine, fertility, neurology and immunology, and oncology.  The life sciences segment of the company offers tools and solutions to support biotechnology and pharmaceutical research; the company claims to have invested EUR 260 million in this segment.  It also specializes in synthesizing high quality, synthetic oligonucleotides (ISO 9001 and ISO 13485 quality systems) for research, diagnostics and therapeutic applications. Further, the performance materials segment of the company is involved in the production of specialty chemicals for diverse applications.  The firm strives to develop products that enhance the ecological footprint, as well as help its clients to meet sustainability goals.

The following table presents the details on the services offered by Merck:

Parameter Description 
Number of Facilities 1
Location of Facilities Massachusetts, US
Type of Oligonucleotide Synthesized 
  • DNA Oligonucleotides
  • Oligonucleotide Probes
  • Primers
  • RNA Oligonucleotides (RNAi)
Scale of Operation
  • Preclinical
  • Clinical
  • Commercial
Application of Area
  • Research
  • Diagnostics
  • Therapeutics
Type of Additional Services Offered
  • Process Development / Method Validation
  • Analytical Testing
  • Custom Synthesis
  • Sequencing
  • Modification
  • Purification
  • Quality Assurance and Quality Control


Recent Initiatives Undertaken by Merck:

  • In January 2022, the company acquired Exelead in order to expand its lipid nanoparticle, liposome, pegylated formulations and oligonucleotide manufacturing capabilities.

Nitto Avecia claims to be amongst the largest manufacturers of oligonucleotides in the world and possess production capacity to meet preclinical, clinical, as well as commercial needs of its customers

Nitto Avecia - Logo

Nitto Avecia is a US-based contract manufacturer, that specializes in the development, manufacturing and modification of a wide range of oligonucleotides. In 2011, Nitto Denko acquired Avecia Biologics in order to expand its contract manufacturing business, specifically in the domain of nucleic acid drugs. In addition to oligonucleotide manufacturing, the company offers analytical testing and method validation, process development, and quality control services to its clients.

The following table presents the details on the services offered by Nitto Avecia:

Parameter Description 
Number of Facilities 2
Location of Facilities
  • Massachusetts, US
  • Ohio, US
Type of Oligonucleotide Synthesized 
  • Antisense Oligonucleotides
  • Aptamers
  • DNA Oligonucleotides
  • RNA Oligonucleotides (miRNA, siRNA and Decoy RNA)
Scale of Operation
  • Preclinical
  • Clinical
  • Commercial
Application of Area Therapeutics
Type of Product Synthesized
  • Oligonucleotide API
  • Finished Dosage Form/ Drug Product
Type of Additional Services Offered
  • Process Development / Method Validation
  • Analytical Testing
  • Custom Synthesis
  • Sequencing
  • Purification
  • Fill-Finish
  • Quality Assurance and Quality Control


Recent Initiatives Undertaken by Nitto Avecia:

  • In February 2022, the company announced the expansion of its oligonucleotide fill-finish capacity up to 1,000 vials, at preclinical scale.

What About the Other Oligonucleotide Synthesis Companies?

The above presentation features 5 oligonucleotide synthesis companies selected from a pool of over 110+ companies that we have compiled. If you're interested, you can download the Sample Report on this topic by Roots Analysis. For personalized assistance in identifying the most relevant solutions based on your specific criteria, please don't hesitate to reach out to us at sales@rootsanalysis.com.

About Author

Jasmeet Bhalla

With an experience of over 4 years with Roots Analysis, Jasmeet is adept at generating useful insights from unstructured / structured datasets. As a senior analyst at Roots Analysis, she has assisted several clients across multiple industry verticals within the healthcare industry. These verticals include contract services, devices / technologies, and drugs / disease indications. Since the findings of the research are aimed at supporting the clients to make thoughtful decisions for their business, she has hands-on experience on competitive landscape assessment, benchmarking, market sizing and forecasting, as well as several quantitative / qualitative / strategic frameworks.

Sources:

1. “Agilent Investing $725 Million to Expand State-of-the-Art Manufacturing Capacity for Production of Nucleic Acid-Based Therapeutics

2. “Ajinomoto Bio-Pharma Services Successfully Develops Highly Functional Ancestral RNA Ligase

3. “Integrated DNA Technologies Opens New Therapeutic Manufacturing Facility to Support Growing Demand in Genomic Medicine

4. “German Merck buys manufacturer of key component in mRNA medicines

5. “Expanded fill-finish capacity to accelerate your preclinical oligonucleotide program

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