Cell Free Protein Expression Market

Cell Free Protein Expression Market: Cell free Expression Kits and Service Providers - Distribution by Type of Cell Free System, Type of Host Organism, Type of Expression Method, End-User and Key Geographical Regions: Industry Trends and Global Forecasts, 2023-2035

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Cell Free Protein Expression Market Overview

The global cell free expression market size is estimated to grow from USD 1 275 million in 2023 to USD 627 million by 2035, representing a CAGR of 7.1% during the forecast period 2023-2035.

In recent years, cell free expression systems have emerged as a promising alternative for the conventional cell based protein manufacturing process. It is a well-known fact that traditional biomanufacturing is a complex process that requires living cells and specific conditions in order to maintain proper homeostasis for cell growth. Moreover, cell based biomanufacturing imposes inherent limitations on the effective production of biologics. On the other hand, cell free protein expression systems are in-vitro platforms that harness the transcription and translation machinery of living cells, in the form of cell lysates, to synthesize various types of biologics, particularly proteins. A number of players have also developed kits for the cell free expression of enzymes, a class of proteins, It is worth highlighting that Enzymit, a bioproduction firm, utilizes cell free technology to synthesize cost-effective enzymes.

Overall, cell free protein expression systems offers several advantages over the traditional process, including the ability to produce a broad range of target molecules by altering components suited for their production, higher yield, shorter development times and low cost of production. Cell free protein expression is carried out using cell free system or commercially available cell free expression kits. Cell-free protein expression systems have demonstrated to be capable of enabling the synthesis of several complex proteins, including toxic proteins, membrane proteins and other post translationally modified proteins, which have been found to be challenging to express using cell based systems. Given the growing demand for biologic products, cell free expression has become a key focus area for stakeholders in the biopharmaceutical industry. We have considered the ongoing activity and existing trends in the cell free protein expression market, including enzymes. Further, we have also looked at the recent trends in the cell free expression of oligonucleotides and other biomolecules, as the cell free trend is catching up beyond protein expression. The global cell free protein expression market is anticipated to witness growth at 7.1% CAGR during the forecast period, driven mainly by the rising demand for protein therapeutics and vaccines.

Key Market Insights

The Cell Free Protein Expression Market: Segmented by Kits and Service Providers - Distribution by Type of Cell Free System (Crude Cell Lysate-Based Systems and Reconstituted Systems), Type of Host Organism (Microbial Cell Free Expression System, Mammalian Cell Free Expression System, Plant-based Cell Free Expression System and Other Cell Free Expression Systems), Type of Expression Method (Coupled Transcription and Translation Method and Translation Method), End-User (Pharmaceutical and Biotechnology Companies, Academic and Research Institutes and Other End-Users) and Key Geographical Regions (North America, Europe, Asia-Pacific and Rest of the World): Industry Trends and Global Forecasts, 2023-2035 report features an extensive market research of the current market landscape of cell free protein expression kits and contract manufacturers / research organizations offering cell free protein expression service, cell free protein expression market size and likely future potential associated with the industry, during the forecast period. Key takeaways of the cell free protein expression market analysis are briefly discussed below.

Cell Free System Market, List of Service

Need for Cell Free Protein Expression

Driven by several blockbuster products and a burgeoning pipeline, biologics (and consequently their manufacturing) have emerged as one of the key focus areas for stakeholders engaged in the pharmaceutical industry. However, biologic manufacturing is often fraught with several challenges, including inconsistencies related to quality of the final product, facility limitations, high attrition rate of pipeline candidates, prolonged development timelines, and regulatory / compliance-related issues. Therefore, biopharmaceutical firms are actively taking initiatives to identify approaches to overcome the existing challenges associated with biologic manufacturing. Amongst several alternatives, the use of various types of cell free protein expression systems, including microbial cell free expression systems, mammalian cell free expression systems, plant-based cell free expression systems and other cell free expression systems, has emerged as a viable option for the synthesis of proteins. This can be attributed to the fact that cell free protein expression systems enable faster and economical synthesis of these complex molecules in shorter time.

Key Advantages of Cell Free Protein Expression

Cell free protein expression systems allow consistent manufacturing of products at varying scales of production (from very small (microliter) to very large (1000 L)), by using the same stockpile of reagents. Additionally, the absence of cell walls in cell free system helps in optimizing various steps of the overall biomanufacturing process by facilitating the addition / removal of enzymes and cofactors (which are continuously produced and degraded at varying rates by living cells), as well as enabling direct monitoring of the entire process. Further, cell free protein expression systems allow rapid manufacturing of time-sensitive products (such as the required therapies / vaccines during a pandemic) from the previously harvested active ribosomes and other necessary cellular machinery.

Cell Free Protein Expression Market Landscape

There are companies offering cell free protein expression kits or cell free protein expression services. The service providers landscape is fragmented, featuring a mix of several large, mid-sized and small companies. It is worth mentioning that, currently, more than 30 players across the world offer a range of cell free protein expression services. Majority of these players are based in the US, followed by those having headquarters in Europe and Asia Pacific. Further, over 90 kits are presently available in the market for cell free expression of proteins. Given the inclination towards cutting-edge technologies, along with innovative approaches to tailor the cell free protein synthesis, we believe that the cell free protein expression market is likely to evolve at a rapid pace, during the forecast period 2023-2035.

Cell Free Protein Expression Market Trends: Partnerships and Collaborations Will Drive the Adoption and Application

Several stakeholders have been forging alliances with other industry / non-industry players in cell free protein expression market for product development, research and development and technology licensing purposes. It is worth highlighting that, since 2018, over 40 strategic partnerships have been inked in the industry. Majority of these agreements were signed for cell-free protein expression of complex proteins, including toxic proteins, membrane proteins and other post translationally modified proteins.

Owing to several advantages of cell free system, the stakeholders are also acquiring other industry players specializing in various aspects of cell free expression / biomanufacturing in order to expand their capabilities and build a comprehensive product / service portfolio. In one such instance, BigHat Biosciences had acquired Frugi Biotechnology in March 2022 to gain access to the latter’s cell free protein synthesis technology. 

Cell Free Protein Expression Market is Driving Developments in the Manufacturing of Other Biomolecules, Including Oligonucleotides

Cell free protein expression system has emerged as potent technology in the field of synthetic biology for high-throughput production of proteins. The success of this platform is also driving the development of cell free systems for expression of other biomolecules such as oligonucleotides (DNA/RNA). Touchlight, a UK based company, claims to manufacture its proprietary dbDNA™ at exceptional speed and large scales using cell free synthesis process. The company claims to use its dbDNA™, as a critical synthesis material for development of DNA and mRNA vaccines, and cell and gene therapies. In January 2023, Moderna acquired OriCiro Genomics, a pioneer in cell free DNA synthesis and amplification technologies, to obtain best-in-class tools for cell free synthesis and amplification of plasmid DNA, a key building block in mRNA manufacturing.

Cell Free Protein Expression Market Size: North America to Hold the Largest Market Share

Owing to the several advantages offered by cell free protein synthesis over the traditional cell-based manufacturing process, the global cell free protein expression market is anticipated to grow at an annualized rate (CAGR) of 7.1% during the forecast period 2023-2035. It is worth highlighting that, in terms of type of cell free protein expression system, the microbial cell free expression system is expected to capture the majority share in the cell-free protein expression market by 2035. Further, amongst the key geographical regions, North America is anticipated to represent the highest share of the cell free protein expression industry in 2035.

Leading Companies in Cell Free Protein Expression Market

Examples of companies providing cell free synthesis and other associated services for proteins include (which have also been captured in this report) BioLinker, CUSABIO and LenioBio. Additionally, examples of players engaged in the development of cell free protein expression kits include (which have also been captured in this report) biotechrabbit, CellFree Sciences, Creative Biolabs, Daicel Arbor Biosciences, Promega and Thermo Fisher Scientific.

Cell Free System Market, List of Cell Free Expression Kits

 

Recent Developments in Cell Free Expression Market

 

Several recent developments have taken place in the field of cell free protein expression. We have outlined some of these recent initiatives below. These developments, even if they took place post the release of our market report, substantiate the overall market trends that have been outlined in our analysis.

  • In December 2023, a study was published on the evaluation of cell free protein expression using BioBits platform aboard the International Space Station (ISS).
  • In August 2023, Enzymit announced successful cell free manufacturing of hyaluronic acid, which can be used in pharmaceutical and cosmetic application.
  • In June 2023, Sutro Biopharma received funding of $140 million from Blackstone Life Sciences

Scope of the Report

The cell free protein expression market report presents an in-depth analysis of the various firms / organizations that are engaged in this market, across different segments, as defined in the below table:

Cell Free Protein Expression Market: Kits and Service Providers, Report Attribute / Market Segmentations

Key Report Attributes

Details

Base Year

2022

Forecast Period

2023-2035

Market Size 2023

$275 million

CAGR

7.1%

Type of Cell Free System

  • Crude Cell Lysate Based Systems
  • Reconstituted systems

Type of Host Organism

  • Microbial cell free expresion systems
  • Mammalian cell free expression systems
  • Plant-based cell free expression system
  • Other cell free expression systems 

Type of Expression Method

  • Coupled transcription and translation method
  • Translation method

End User

  • Pharmaceutical and Biotechnology Companies
  • Academic and Research Institutes
  • Other End Users

Key Geographical Regions

  • North America
  • Europe
  • Asia-Pacific
  • Rest of the World

Example Companies Profiled

  • BioLinker
  • Biotechrabbit
  • CellFreeSciences
  • Creative Biolabs
  • CUSABIO
  • Daicel Arbor Biosciences
  • LenioBio
  • Promega
  • Thermo Fisher Scientific
  • Touchlight

Customizatiion Scope

15% Free Customization Option (equivalent to 5 analysts working days)

PowerpPoint Presentation (Complimentary)

Available

Excel Data Packs

  • Market Landscape Analysis
  • Company Competitiveness Analysis
  • Product Competitiveness Analysis
  • Partnerships and Collaborations Analysis
  • Funding and Investment Analysis
  • Patent Analysis
  • Market Forecast and Opportunity Analysis

 

 

Cell Free System Market, Distribution by Type of Cell Free System

The cell free protein expression market research report presents an in-depth analysis, highlighting the capabilities of various stakeholders engaged in this market, across different geographies. Amongst other elements, the report includes:

  • An executive summary of insights captured during our research. It offers a high-level view on the current state of the cell free protein expression market and its likely evolution in the given forecast period.
  • A general overview of cell free systems, the different types of such systems and their composition. It provides a brief outline of the cell free expression process, highlighting several challenges and advantages associated with this technique. It also features applications of cell free expression in the healthcare industry, as well as other industries and a discussion on future prospects of cell free expression.
  • A detailed assessment of the current market landscape of cell free protein expression service providers, based on several relevant parameters, such as year of establishment, company size (in terms of employee count), location of headquarters, type of cell free service(s) offered (biomolecule synthesis / expression, template construction, scaled-up production, process optimization and feasibility testing), type of associated service(s) offered (purification, quality control and others), type of host organism (E. coli, wheat germ, HeLa / human cell lysate, rabbit reticulosyte, Nicotiana tabacum, Spodoptera frugiperda, Chinese hamster ovary, rice cell system, Saccharomyces cerevisiae  and silkworm), type of product(s) synthesized (membrane proteins, toxic proteins, proteins with disulfide bonds / antibodies, protein complexes, soluble proteins, enzymes, modified proteins, antigens, insoluble proteins, high molecular weight proteins, other proteins, and unspecified proteins), scale of operation (lab scale / pre-clinical, clinical and commercial) and type of industry served (pharmaceutical and biotechnology, academics, diagnostics, chemicals and food). Also included are companies providing service for cell free synthesis of oligonucleotides.
  • A detailed assessment of the current market landscape of cell free protein expression kits, based on several relevant parameters, such as type of cell free system (crude cell lysate-based systems and reconstituted systems), type of host organism (microbial cell free expression system, plant-based cell free expression system, mammalian cell free expression system and insect-based cell free expression system), type of expression method (coupled transcription and translation method and translation method), type of product(s) synthesized (toxic proteins, soluble proteins, membrane proteins, labeled proteins, functionally active proteins, proteins with disulfide bonds, proteins with unnatural amino acids, high molecular weight proteins, insoluble proteins, heat-sensitive proteins, enzymes, other proteins, and unspecified proteins), type of template(s) used (circular DNA, linear DNA, RNA and others), type of compatible promoter(s) (T7, SP6 and others), type of reaction format (batch format, bilayer format, CECF format, and batch and CECF format), yield of positive control (1-5 µg/mL, >5-100 µg/mL, >100-200 µg/mL, >200-500 µg/mL, >500-1,000 µg/mL, >1,000-2,000 µg/mL, >2,000-3,000 µg/mL and >3,000 µg/mL), reaction volume (1-5 µL, >5-100 µL, >100-200 µL, >200-500 µL, >500-1,000 µL, >1,000-2,000 µL, >2,000-3,000 µL, >3,000-10,000 µL and >10,000 µL), reaction temperature (20°C-25°C, >25°C-30°C, >30°C-35°C and >35°C-40°C),  incubation time (1-2 hours, >2-10  hours, >10-20  hours and >20  hours), number of reactions per kit (1-5 reactions / kit, >5-20 reactions / kit, >20-50 reactions / kit, >50-80 reactions / kit and >80-100 reactions / kit), price per kit (USD 150-200, USD >200-500, USD >500-1,000, USD >1,000-2,000, USD >2,000-3,000, USD >3,000-4,000, USD >4,000-5,000 and USD >5,000). In addition, it provides details on cell free protein expression kits developers, along with analyses based on their year of establishment, company size (in terms of employee count), location of headquarters and leading players (in terms of number of kits provided). Also included are companies providing kits for cell free synthesis of oligonucleotides and whole cells.
  • A comprehensive company competitiveness analysis of cell free expression service providers based on supplier power (in terms of number of years of experience) and service strength (in terms of type of host organism, service(s) offered, number of product(s) synthesized, industries served and scale of operation).
  • A product competitiveness analysis of cell free expression kits based on various relevant parameters, namely developer power (in terms of number of years of experience) and product competitiveness (in terms of type of cell free system, type of expression method, type of host organism, number of product(s) synthesized, number of template(s) used, number of compatible promoter(s), yield of positive control (µg/ml), reaction volume (µl), incubation time (hours), type of reaction format, reaction temperature and number of reactions per kit).
  • Elaborate profiles of prominent players (shortlisted based on a proprietary criterion) offering cell free expression services. Each profile features a brief overview of the company, details on its cell free expression service portfolio, recent developments and an informed future outlook.
  • Elaborate profiles of prominent players engaged in development of cell free expression kits (shortlisted based on a proprietary criterion), featuring a brief overview of the company, details on its product portfolio, recent developments and an informed future outlook.
  • A detailed analysis of recent partnerships inked between stakeholders engaged in this industry, since 2018, covering product development agreements, research and development agreements, technology licensing agreements, acquisitions, product licensing agreements, service alliances, asset acquisitions, joint ventures, product development and commercialization agreements, technology utilization agreements and other relevant agreements. 
  • An analysis of the funding and investments made in the market, since 2018, including seed financing, venture capital financing, capital raised from IPOs, secondary offerings, grants / awards, private placements, other equity and debt financing, at various stages of development in companies that are engaged in this field, based on several parameters, such as number of funding instances, amount invested, type of funding, leading players and investors, and geographical analysis.
  • An in-depth analysis of various patents that have been filed / granted related to cell free system since 2015, taking into consideration several relevant parameters, such as patent publication year, type of patent, patent jurisdiction, CPC symbols, type of applicant, emerging focus areas, leading players (in terms of number of patents filled / granted) and leading individual assignees. It also features a detailed patent benchmarking analysis and an insightful valuation analysis, highlighting leading patents (in terms of number of citations).

One of the key objectives of this market report was to estimate the current cell free protein expression market size, opportunity and the future growth potential of the kits and service providers, over the forecast period. We have provided informed estimates on the likely evolution of the market for the forecast period, 2023-2035. Our year-wise projections of the current and forecasted opportunity have been further segmented based on relevant parameters, such as Type of Cell Free System (Crude Cell Lysate-Based Systems and Reconstituted Systems), Type of Host Organism (Microbial Cell Free Expression System, Mammalian Cell Free Expression System, Plant-based Cell Free Expression System and Other Cell Free Expression Systems), Type of Expression Method (Coupled Transcription and Translation Method and Translation Method), End-User (Pharmaceutical and Biotechnology Companies, Academic and Research Institutes and Other End-Users) and Key Geographical Regions (North America, Europe, Asia-Pacific and Rest of the World). In order to account for future uncertainties associated with some of the key parameters and to add robustness to our model, we have provided three market forecast scenarios, namely conservative, base and optimistic scenarios, representing different tracks of the market growth.

The opinions and insights presented in the report were influenced by discussions held with stakeholders in this industry. The report features detailed transcripts of interviews held with the following individuals (in alphabetical order of company names):

  • Chief Operational Officer, Small Company, Japan
  • Chief Executive Officer and Founder, Small Company, Germany
  • Chief Executive Officer and Co-founder, Small Company, France

All actual figures have been sourced and analyzed from publicly available information forums and primary research discussions. Financial figures mentioned in this report are in USD, unless otherwise specified.

Frequently Asked Questions

Question 1: What is a cell free system?

Answer: Cell free system is an in-vitro platform which allows the occurrence of biochemical reactions in the absence of living cells.

Question 2: How does cell-free protein synthesis work?

Answer: Cell free systems employ the bio machinery harvested from the lysate of disrupted cells for the manufacturing of a wide array of macromolecular products, mainly proteins.

Question 3: How big is the cell free protein expression market?

Answer: The cell free protein expression market size is estimated to be $275 million in 2023.

Question 4: What is the likely cell free protein expression market growth rate (CAGR)?

Answer: The cell free protein expression market is expected to grow at a CAGR of 7.1% during the forecast period.

Question 5: Who are the leading companies offering cell free expression services?

Answer: Examples of key companies currently offering cell free expression services include BioLinker, CusaBio, LenioBio and Touchlight

Question 6: Who are the leading companies offering cell free expression kits globally?

Answer: Examples of leading companies currently providing cell free expression kits include biotechrabbit, CellFree Sciences, Creative Biolabs, Daicel Arbor Biosciences, Promega and Thermo Fisher Scientific.

Question 7: What are the partnership trends observed related to cell free protein expression market?

Answer: Product development agreements, research and development agreements, technology licensing agreements, and acquisitions are the most common types of partnerships inked by stakeholders engaged in this market.

Question 8: Which region captures the largest share of the cell free protein expression market?

Answer: North America captures around 55% share in the current market. It is also worth highlighting that the cell free protein expression market in Asia-Pacific is anticipated to grow at a relatively faster pace.

Question 9: Which segments represent the largest share of cell free protein expression market?

Answer: Currently, in terms of type of cell free system, crude cell lysate-based systems capture the largest share of the overall market. However, the market for reconstituted systems is likely to grow at relatively faster pace in the long term.

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