Since the introduction of a gene sequencing method by Frederick Sanger in 1977, the field of genomic data collection and analysis has evolved significantly. Advancement in DNA sequencing technologies have resulted in noteworthy developments in various healthcare-related research fields, such as diagnostics and personalized medicine. However, the conventional nucleotide library preparation process has several challenges, including requirement of huge amount of starting materials, inadequate throughput and deteriorated quality of the product. Moreover, the manual protocols require extensive manipulation, costly reagents and long duration of skilled genomic library production. As a result, NGS library preparation kits have emerged as an innovative technology to overcome the challenges associated with conventional library preparation protocols. These kits require less input sample, produce sufficient DNA / RNA libraries with minimum errors and are compatible with a broad range of input samples, including blood, cells, plasma and saliva. It is important to highlight that several companies are providing NGS library preparation kits to identify the novel COVID-19 virus and monitor its mutation events.
Advantages of Next Generation Sequencing
NGS is a renowned tool in functional genomics with numerous opportunities in clinical practice. The various advantages of NGS are briefly described below:
Diverse Landscape of NGS Library Preparation Kits
During our research, we came across more than 55 players, which claim to offer 280+ NGS library preparation kits. As per our analysis, majority of the kits use purified RNA / DNA as the input sample. This is followed by kits that use other starting materials, such as bacterial colonies, formalin-fixed paraffin-embedded (FFPE) tissue, fresh frozen tissue, hair and tongue swabs.
Product Competitiveness Analysis
Several NGS library preparation kit providers are constantly making efforts to expand current capabilities to enhance their respective product portfolios and comply to evolving industry benchmarks.
NGS Service Providers
Approximately 70 industry players claim to provide various NGS based services, supporting a variety of second and third generation sequencing technologies. As per our analysis, majority of these players are located in North America, followed by Asia Pacific.
The evolution of NGS technologies has made it possible to sequence thousands of genes with a suspected genetic disease predisposition, in a very short period of time and at minimal costs. Over the years, several established players, as well as start-ups, have emerged in this domain to offer various types of NGS kits for library preparation of nucleotides. Driven by the increasing demand for gene therapies, favourable regulatory scenario and reduced cost of sequencing, the demand for NGS library preparation kits and reagents are likely to increase in the coming years.
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